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و این هم خلاصه مقاله دکتر سالکده:
Molecular Analysis of Human Embryonic Stem Cells Differentiation to Neural Cells: a Quantitative Proteome and Transcriptome approaches
Ali Fathi1, Haghighat Vakilian1, Leila Hatami2, Chia Li Han3, Yu-Ju Chen3, Hossein Baharvand2, Ghasem Hosseini Salekdeh1
1Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2Department of Stem Cells, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Institute of Chemistry, Academia Sinica, Taipei, Taiwan
salekdeh@royaninstitute.org“> salekdeh@royaninstitute.org
Molecular analysis of neural differentiation of human embryonic stem cells (hESCs) provides a unique view on of the molecular mechanisms involved in neurogenesis. Using a microarray- and DIGE-based transcriptome and proteome approaches, respectively, we studied hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and differentiated neurons. Time-dependent gene expression patterns of early hESC differentiation were analyzed and the function of two genes, MAGOHB and BCAS2, which showed changes at both at RNA and protein levels during differentiation was further validated using siRNA approach. The knock down of both genes resulted in an increase in the expression of neural progenitor markers such as Pax6 and Nestin. Furthermore, the silencing of BCAS2 led to a decrease in Nanog expression level and an increase in the abundance of P53. The down-regulation of BCAS2 might enhance neurogenesis through interaction and negative regulation of P53 expression. The suppression of MAGOHB resulted in down-regulation of early endoderm and mesodermal gene markers, suggesting that it may play a role in enhancing neurogenesis through inhibition of endo-mesodermal formation. Overall, these results enhanced our knowledge about the molecular changes accompanying neural commitment and differentiation.
Keywords:
Proteomics/ microarray/Embryonic stem cells/ neural cells/ Human
References
- 1.Fathi, A., Hatami, M., Hajihosseini, V., Fattahi, F., Kiani, S., Baharvand, H., Salekdeh,
G. H., PLoS One 201, 6:e22856.
2. Reiland, S., Salekdeh, G.H., Krijgsveld, J., Expert Rev Proteomics 2011, 8, 29-42
3. Baharvand, H., Fathi, A., van Hoof, D., Salekdeh, G.H., Stem Cells 2007, 25, 1888-903.